Journal: Journal of Cellular and Molecular Medicine

Article Title: E10A, an adenovirus carrying human endostatin gene, in combination with docetaxel treatment inhibits prostate cancer growth and metastases

doi: 10.1111/j.1582-4934.2008.00548.x

Figure Lengend Snippet: (A) LYVE-1-positive lymphatic vessels in orthotopic tumours received the different treatment. Representative images were shown (×200). (B) Serum endostatin levels in the mice with orthotopic tumours. After systemic delivery of E10A, serum endostatin levels were determined by ELISA. Serum endostatin levels in mice treated with Ad-LacZ were used as control. (C) Serum VEGF levels in different groups of mice. Mice were killed after 4 weeks of treatment and serum were collected for determination of VEGF levels by ELISA. Columns, mean ± S.D. * P < 0.05, compared with Ad-LacZ control.

Article Snippet: The supernatants of PC-3 cells after 72 hrs of infection with E10A or Ad-LacZ at MOI of 100 were harvested as conditioned medium (CM) and evaluated endostatin protein using a human endostatin ELISA kit (R&D systems, Minneapolis, MN, USA) according to the recommendation of the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Tissue Engineering. Part A

Article Title: Engineering Endostatin-Producing Cartilaginous Constructs for Cartilage Repair Using Nonviral Transfection of Chondrocyte-Seeded and Mesenchymal-Stem-Cell-Seeded Collagen Scaffolds

doi: 10.1089/ten.tea.2009.0771

Figure Lengend Snippet: Endostatin in the medium of cell-seeded, lipoplex-supplemented collagen constructs and control constructs. (a) Mesenchymal-stem-cell (MSC)-seeded type I/III constructs cultured in chondrogenic medium (CM) and nonchondrogenic medium (NCM) (n = 3 for first 6 days of lipoplex-supplemented scaffolds; n = 2 for all others). (b) Modification of the scaffold via crosslinking none, half, or all of the lipoplexes (n = 6), in MSC-seeded type I/III constructs cultured in CM. (c) MSCs cultured at standard and low oxygen in type I/III and type II collagen scaffolds in CM (n = 3). (d) Chondrocytes cultured at standard and low oxygen in type I/III and type II collagen scaffolds in CM (n = 3).

Article Snippet: Endostatin detection in the medium Endostatin protein in the culture medium was measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit for human endostatin protein (R&D Systems) following the manufacturer's instructions.

Techniques: Construct, Control, Cell Culture, Modification

Journal: Tissue Engineering. Part A

Article Title: Engineering Endostatin-Producing Cartilaginous Constructs for Cartilage Repair Using Nonviral Transfection of Chondrocyte-Seeded and Mesenchymal-Stem-Cell-Seeded Collagen Scaffolds

doi: 10.1089/ten.tea.2009.0771

Figure Lengend Snippet: Micrographs of histological sections of chondrocyte- and MSC-seeded constructs cultured in CM and NCM at standard oxygen. (a) Endostatin immunohistochemistry of MSC-seeded CI scaffold supplemented with 20 μg pEndo after 6 days of culture in NCM (red chromogen [arrows] indicates positive stain). Scale bar, 50 μm, 40 × magnification. (b) Hematoxylin and eosin stain of the interior of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM. Scale bar, 50 μm, 40 × magnification. Inset shows the section at lower magnification, scale bar, 100 μm, 10 × magnification. (c) Hematoxylin and eosin stain of the surface of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM. Scale bar, 100 μm, 10 × magnification. (d) Safranin-O staining of glycosaminoglycan (GAG) for MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM (red chromogen indicates sulfated GAGs). Scale bar, 200 μm, 10× magnification. (e) Type II collagen immunohistochemistry of MSC-seeded CI scaffold supplemented with 4 μg pEndo after 22 days of culture in CM (red indicates positive stain). Scale bar, 200 μm, 10 × magnification. (f) Safranin-O staining of GAG for chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (red chromogen indicates sulfated GAGs). Scale bar, 50 μm, 40 × magnification. Inset shows the section at lower magnification, scale bar, 500 μm, 4 × magnification. (g) Type II collagen immunohistochemistry of chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (brownish-red chromogen indicates positive stain). Scale bar, 500 μm, 4 × magnification. (h) Masson's trichrome staining of chondrocyte-seeded CI scaffold supplemented with 20 μg pEndo after 20 days of culture in CM (blue chromogen indicates collagen, red chromogen indicates cytoplasm, and black chromogen indicates nuclei). Scale bar, 500 μm, 4 × magnification. Color images available online at www.liebertonline.com/ten.

Article Snippet: Endostatin detection in the medium Endostatin protein in the culture medium was measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit for human endostatin protein (R&D Systems) following the manufacturer's instructions.

Techniques: Construct, Cell Culture, Immunohistochemistry, Staining, H&E Stain